Test Procedures for Insecticide Resistance Monitoring in Malaria Vector Mosquitoes

This publication:

• provides background information on how the WHO susceptibility test has evolved leading to the current revision;

• focusing on adult mosquito testing, it provides details on discriminating concentrations of the insecticides to be tested, as well as recommended equipment and supplies, sampling protocols, test conditions and protocols;

• explains how susceptibility test results are recorded and reported, i.e. calculation of mortality and knock-down rates, interpretation of susceptibility test results, reporting results of susceptibility testing;

• where appropriate, recommends further investigations i.e. identification of resistance mechanisms such as biochemical enzyme assays, molecular (biological) tests and synergist assays; and

• for the first time the CDC bottle assay, an alternative susceptibility test – has been included in the test procedures.
One of the most interesting features of this publication is on the reporting of test results in line with GPIRM recommendations for pre-emptive action in managing insecticide resistance.